@article{ross_multipurpose_2024,

title = {Multipurpose On-the-Spot Peptide-Based Hydrogels for Skin, Cornea, and Heart Repair},

author = {Alex Ross and Xixi Guo and German A. Mercado Salazar and Sergio David Garcia Schejtman and Jinane El-Hage and Maxime Comtois-Bona and Aidan Macadam and Irene Guzman-Soto and Hiroki Takaya and Kevin Hu and Bryan Liu and Ryan Tu and Bilal Siddiqi and Erica Anderson and Marcelo Muñoz and Patricio Briones-Rebolledo and Tianqin Ning and May Griffith and Benjamin Rotsein and Horacio Poblete and Jianyu Li and Marc Ruel and Erik J. Suuronen and Emilio I. Alarcon},

url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.202402564},

doi = {https://doi.org/10.1002/adfm.202402564},

year  = {2024},

date = {2024-01-01},

journal = {Advanced Functional Materials},

volume = {34},

number = {37},

pages = {2402564},

abstract = {Abstract Bioinspired synthetic materials can be designed as reliable, cost-effective, and fully controlled alternatives to natural biomaterials for treating damaged tissues and organs. However, several hurdles need to be overcome for clinical translation, particularly for biomaterials gelled in situ. These include the potential toxicity of chemical crosslinkers used in the materials' assembly or breakdown products they generate and the challenges of fine-tuning the mechanical properties of the materials. Here, a minimalistic, adhesive soft material is developed by screening hundreds of potential formulations of self-assembling, custom-designed collagen-like peptide sequences for the in situ formation of tissue-bonding 3D hydrogels. Nine promising formulations for tissue repair are identified using a low-volume and rapid combinatory screening approach. It is shown that simply varying the ratio of the two key components promotes adhesion and fine-tunes the material's mechanical properties. The materials' skin and heart repair capabilities are assessed in vitro and clinically relevant animal models. The materials are also tested for corneal applications using ex vivo pig cornea models complemented by in vitro cell compatibility assays.},

note = {_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/adfm.202402564},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zuniga-nunez_time-resolved_2023,

title = {Time-resolved fluorescence and anisotropy-sensitive 1,2-dimyristoyl-sn-glycero-3-(7-aminocoumarin) phosphoetanolamide probe for studying membrane lipid domains},

author = {Daniel Zúñiga-Núñez and Francisco Mura and Nory Mariño-Ocampo and Patricio Briones-Rebolledo and Horacio Poblete and Jean-Maurice Mallet and Denis Fuentealba and Alexis Aspée},

url = {https://www.sciencedirect.com/science/article/pii/S0143720823002942},

doi = {https://doi.org/10.1016/j.dyepig.2023.111368},

issn = {0143-7208},

year  = {2023},

date = {2023-01-01},

journal = {Dyes and Pigments},

volume = {217},

pages = {111368},

abstract = {A fluorescent probe C-DMPE was synthesised to monitor interfacial membrane properties by conjugating coumarin-343 and 1,2-dimyristoyl-sn-glycero-3-phosphorylethanolamine (DMPE), anchoring the 7-aminocoumarin moiety close to the phospholipid polar head at the membrane interface. Large unilamellar vesicles (LUV) of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC), of 1,2-dioleyl-sn-glycero-3-phosphatidylcholine (DOPC) and cholesterol were employed as a model of lipid bilayer. Time-resolved fluorescence developed an emissive Internal Charge Transfer excited state with a long fluorescence lifetime (τ1), a Locally Excited state with an intermediate fluorescence lifetime (τ2), and a short lifetime (τ3) associated with an intermolecular quenching by interaction with a phosphate group of neighbour phospholipids, as is clearly shown by molecular dynamics simulations. Shorter values of fluorescence lifetimes τ1 and τ3 were observed in DOPC with respect to DPPC, responding to a more fluid membrane with more significant water accessibility in DOPC than DPPC. However, in DPPC:DOPC vesicles, these fluorescence lifetimes are even shorter, allowing to be attributed to favourable sensing of boundary limit lipid domains. In similitude, time-resolved anisotropy showed shorter rotational correlation times φ1, in DPPC: DOPC vesicles than in DOPC associated with a faster internal rotational movement of the 7-aminocoumarin group in domains than in fluid a DOPC membrane. In addition, shorter rotational correlation times, φ2, were also observed in DPPC:DOPC vesicles compared to DPPC, suggesting a faster lateral diffusion of the probe in the presence of domains.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}