@article{nokey,

title = {Dynamics of homeostats: the basis of electrical, chemical, hydraulic, pH and calcium signaling in plants},

author = {Leslie Contador-Álvarez and Tamara Rojas-Rocco and Talía Rodríguez-Gómez and María Eugenia Rubio-Meléndez and Janin Riedelsberger and Erwan Michard and Ingo Dreyer},

doi = {10.1017/qpb.2025.6},

year  = {2025},

date = {2025-03-21},

urldate = {2025-03-21},

abstract = {Homeostats are important to control homeostatic conditions. Here, we have analyzed the theoretical basis of their dynamic properties by bringing the K homeostat out of steady state (i) by an electrical stimulus, (ii) by an external imbalance in the K+ or H+ gradient or (iii) by a readjustment of transporter activities. The reactions to such changes can be divided into (i) a short-term response (tens of milliseconds), where the membrane voltage changed along with the concentrations of ions that are not very abundant in the cytosol (H+ and Ca2+), and (ii) a long-term response (minutes and longer) caused by the slow changes in K+ concentrations. The mechanistic insights into its dynamics are not limited to the K homeostat but can be generalized, providing a new perspective on electrical, chemical, hydraulic, pH and Ca2+ signaling in plants. The results presented here also provide a theoretical background for optogenetic experiments in plants.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Muñoz-Grez2025,

title = {Host-microbe computational proteomic landscape in oral cancer revealed key functional and metabolic pathways between Fusobacterium nucleatum and cancer progression},

author = {Camila Paz Muñoz-Grez and Mabel Angélica Vidal and Tamara Rojas-Rocco and Luciano Esteban Ferrada and Felipe Andrés Zuñiga and Agustin Andrés Vera and Sergio Andrés Sanhueza and Romina Andrea Quiroga and Camilo Daniel Cabrera and Barbara Evelyn Antilef and Ricardo Andrés Cartes and Milovan Paolo Acevedo and Marco Andrés Fraga and Pedro Felipe Alarcón-Zapata and Mauricio Alejandro Hernández and Alexis Marcelo Salas-Burgos and Francisco Tapia-Belmonte and Milly Loreto Yáñez and Erick Marcelo Riquelme and Wilfredo Alejandro González and Cesar Andrés Rivera and Angel Alejandro Oñate and Liliana Ivonne Lamperti and Estefanía Nova-Lamperti },

doi = {10.1038/s41368-024-00326-8},

year  = {2025},

date = {2025-01-02},

urldate = {2025-01-02},

abstract = {Oral squamous cell carcinoma (OSCC) is the most common manifestation of oral cancer. It has been proposed that periodontal pathogens contribute to OSCC progression, mainly by their virulence factors. However, the main periodontal pathogen and its mechanism to modulate OSCC cells remains not fully understood. In this study we investigate the main host-pathogen pathways in OSCC by computational proteomics and the mechanism behind cancer progression by the oral microbiome. The main host-pathogen pathways were analyzed in the secretome of biopsies from patients with OSCC and healthy controls by mass spectrometry. Then, functional assays were performed to evaluate the host-pathogen pathways highlighted in oral cancer. Host proteins associated with LPS response, cell migration/adhesion, and metabolism of amino acids were significantly upregulated in the human cancer proteome, whereas the complement cascade was downregulated in malignant samples. Then, the microbiome analysis revealed large number and variety of peptides from Fusobacterium nucleatum (F. nucleatum) in OSCC samples, from which several enzymes from the L-glutamate degradation pathway were found, indicating that L-glutamate from cancer cells is used as an energy source, and catabolized into butyrate by the bacteria. In fact, we observed that F. nucleatum modulates the cystine/glutamate antiporter in an OSCC cell line by increasing SLC7A11 expression, promoting L-glutamate efflux and favoring bacterial infection. Finally, our results showed that F. nucleatum and its metabolic derivates promote tumor spheroids growth, spheroids-derived cell detachment, epithelial-mesenchymal transition and Galectin-9 upregulation. Altogether, F. nucleatum promotes pro-tumoral mechanism in oral cancer.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Zúñiga2022,

title = {A Direct Interaction between Cyclodextrins and TASK Channels Decreases the Leak Current in Cerebellar Granule Neurons},

author = {Rafael Zúñiga and Daniel Mancilla and Tamara Rojas-Rocco and Fernando Vergara and Wendy González and Marcelo A. Catalán and Leandro Zúñiga },

doi = {10.3390/biology11081097},

year  = {2022},

date = {2022-07-22},

urldate = {2022-07-22},

abstract = {Two pore domain potassium channels (K2P) are strongly expressed in the nervous system (CNS), where they play a central role in excitability. These channels give rise to background K+ currents, also known as IKSO (standing-outward potassium current). We detected the expression in primary cultured cerebellar granule neurons (CGNs) of TWIK-1 (K2P1), TASK-1 (K2P3), TASK-3 (K2P9), and TRESK (K2P18) channels by immunocytochemistry and their association with lipid rafts using the specific lipids raft markers flotillin-2 and caveolin-1. At the functional level, methyl-β-cyclodextrin (MβCD, 5 mM) reduced IKSO currents by ~40% in CGN cells. To dissect out this effect, we heterologously expressed the human TWIK-1, TASK-1, TASK-3, and TRESK channels in HEK-293 cells. MβCD directly blocked TASK-1 and TASK-3 channels and the covalently concatenated heterodimer TASK-1/TASK-3 currents. Conversely, MβCD did not affect TWIK-1- and TRESK-mediated K+ currents. On the other hand, the cholesterol-depleting agent filipin III did not affect TASK-1/TASK-3 channels. Together, the results suggest that neuronal background K+ channels are associated to lipid raft environments whilst the functional activity is independent of the cholesterol membrane organization.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zuniga_direct_2022,

title = {A Direct Interaction between Cyclodextrins and TASK Channels Decreases the Leak Current in Cerebellar Granule Neurons},

author = {Rafael Zúñiga and Daniel Mancilla and Tamara Rojas-Rocco and Fernando Vergara and Wendy González and Marcelo A. Catalán and Leandro Zúñiga},

url = {http://dx.doi.org/10.3390/biology11081097},

doi = {10.3390/biology11081097},

issn = {2079-7737},

year  = {2022},

date = {2022-07-01},

urldate = {2022-07-01},

journal = {Biology},

volume = {11},

number = {8},

pages = {1097},

note = {Publisher: MDPI AG},

keywords = {},

pubstate = {published},

tppubtype = {article}

}